100nm NHS-Activated Gold Nanoparticle Conjugation Kit (10 Reactions) With Credit Card Cheap Pice

Cytodiagnostics 100nm NHS-activated Gold Nanoparticle Conjugation Kit have been optimized for high efficiency one-step covalent conjugation of proteins and other primary amine-containing ligands to gold nanoparticles.

The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the gold nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester gold nanoparticles supplied in the kit. 

This mini kit contains 10 innavidual reaction vials with lyophilized NHS-activated gold nanoparticles for performing ten independent conjugation reactions.

Kit Components

• 100nm NHS-Activated Gold Nanoparticles (lyophilized) – 10 vials
• Protein Resuspension Buffer – 1.5ml
• Reaction Buffer – 1.5ml
• Quencher Solution – 1.5ml

Features & Benefits

• Results in covalently bound ligand and more stable conjugate.
• Fast and convenient one-step conjugation reaction with no pre-activation requirements.
• Spacer between the gold nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
• Precisely engineering surface coating that reduces non-specific protein binding in biological assays.

Gold Nanoparticle Specifications

Surface functionality: NHS-ester (~ 1 NHS group/nm2) (10kDa PEG-spacer between gold surface and NHS-group)

Core diameter: 100nm 

Concentration: OD=20 (when dissolved to a final volume of 100ul)

Particles per ml: ~7.68E+10 (when dissolved to a final volume of 100ul)

Absorbance (lambda max): 572nm

Product Data

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Diameter  Peak SPR Wavelength (nm) NPS/ml* Wt. Conc. (mg/ml)* Molar Ext. (M-1cm-1) Size Dispersity (+/-nm) Particle Volume (nm3) Surface Area (nm2) Surface/Volume Ratio Particle Mass (g) Molar Mass (g/mol) Molar Concentration* 100 572 3.84E+09 3.89E-02 1.57E+11 <8% 5.24E+05 3.14E+04 0.06 1.02E-14 6.11E+09 6.37E-12

* All concentrations as indicated at an optical density of 1 (OD=1, i.e. absorbance at peak SPR wavelength with a 1cm pathlength)

 

Product Sheet & SDS

 Product Sheet

 Safety Data Sheet (SDS)

QC Conjugation Kit – Product Sheet